Carrying life and death: the daily routine of hospital stretcher bearers in the context of COVID-19. / Carregando vidas e mortes: o cotidiano de trabalho de maqueiros hospitalares no contexto da COVID-19.

This article aims to analyze the daily work of stretcher bearers in a Federal Teaching and Research Hospital in the context of COVID-19, exploring the subjective aspects related to working in constant contact with death and the invisibility to which these workers are subjected. The proposed discussion stems from a more comprehensive qualitative investigation. The main methodological resource of the empirical research was the direct observation in the daily work that allowed one to capture nuances of the work in a dialectical analysis with the workers. The data obtained were analyzed from the perspective of Social Psychology of Work. Transience and invisibility were identified as important categories of analysis of this daily work, where the process of becoming a stretcher-bearer was marked by aspects of precariousness of work and vulnerability of the worker. Working in contact with death was also understood as a factor of invisibility, where the creation of bonds and humor appeared as a collective strategy to face the harshness of work. This article concluded by examining the importance of looking at the subjective aspects of the work carried out by stretcher bearers, as well as the expansion of research on the subject.

Este artigo analisa o cotidiano de trabalho de maqueiros em um Hospital Federal de Ensino e Pesquisa no contexto da COVID-19, explorando os aspectos subjetivos referentes ao trabalhar em constante contato com a morte e à invisibilidade a que esses trabalhadores estão submetidos. Adotamos o referencial da Psicologia Social do Trabalho, como técnica de pesquisa foram feitas observação direta no cotidiano de trabalho e rodas de conversa com os maqueiros, o que permitiu capturar nuances do trabalho numa análise dialética junto aos trabalhadores. A transitoriedade e invisibilidade foram identificadas enquanto importantes categorias de análise desse cotidiano trabalho, onde o processo de tornar-se maqueiro era marcado por aspectos de precarização do trabalho e vulnerabilização do trabalhador. O trabalho em contato com a morte também foi compreendido como um fator de invisibilidade, onde a criação de vínculos e humor emergem como estratégias coletiva de enfrentamento da dureza inerente ao trabalho. Conclui-se a importância de olhar para os aspectos subjetivos do trabalho realizado pelos maqueiros, assim como a ampliação de pesquisas sobre a saúde desses trabalhadores.

Carregando vidas e mortes: o cotidiano de trabalho de maqueiros hospitalares no contexto da COVID-19 / Carrying life and death: the daily routine of hospital stretcher bearers in the context of COVID-19

Resumo Este artigo analisa o cotidiano de trabalho de maqueiros em um Hospital Federal de Ensino e Pesquisa no contexto da COVID-19, explorando os aspectos subjetivos referentes ao trabalhar em constante contato com a morte e à invisibilidade a que esses trabalhadores estão submetidos. Adotamos o referencial da Psicologia Social do Trabalho, como técnica de pesquisa foram feitas observação direta no cotidiano de trabalho e rodas de conversa com os maqueiros, o que permitiu capturar nuances do trabalho numa análise dialética junto aos trabalhadores. A transitoriedade e invisibilidade foram identificadas enquanto importantes categorias de análise desse cotidiano trabalho, onde o processo de tornar-se maqueiro era marcado por aspectos de precarização do trabalho e vulnerabilização do trabalhador. O trabalho em contato com a morte também foi compreendido como um fator de invisibilidade, onde a criação de vínculos e humor emergem como estratégias coletiva de enfrentamento da dureza inerente ao trabalho. Conclui-se a importância de olhar para os aspectos subjetivos do trabalho realizado pelos maqueiros, assim como a ampliação de pesquisas sobre a saúde desses trabalhadores.

Abstract This article aims to analyze the daily work of stretcher bearers in a Federal Teaching and Research Hospital in the context of COVID-19, exploring the subjective aspects related to working in constant contact with death and the invisibility to which these workers are subjected. The proposed discussion stems from a more comprehensive qualitative investigation. The main methodological resource of the empirical research was the direct observation in the daily work that allowed one to capture nuances of the work in a dialectical analysis with the workers. The data obtained were analyzed from the perspective of Social Psychology of Work. Transience and invisibility were identified as important categories of analysis of this daily work, where the process of becoming a stretcher-bearer was marked by aspects of precariousness of work and vulnerability of the worker. Working in contact with death was also understood as a factor of invisibility, where the creation of bonds and humor appeared as a collective strategy to face the harshness of work. This article concluded by examining the importance of looking at the subjective aspects of the work carried out by stretcher bearers, as well as the expansion of research on the subject.

Antifungal Activity of Guiera senegalensis: From the Chemical Composition to the Mitochondrial Toxic Effects and Tyrosinase Inhibition.

Pest resistance against fungicides is a widespread and increasing problem, with impact on crop production and public health, making the development of new fungicides an urgent need. Chemical analyses of a crude methanol extract (CME) of Guiera senegalensis leaves revealed the presence of sugars, phospholipids, phytosterols, guieranone A, porphyrin-containing compounds, and phenolics. To connect chemical composition with biological effects, solid-phase extraction was used to discard water-soluble compounds with low affinity for the C18 matrix and obtain an ethyl acetate fraction (EAF) that concentrates guieranone A and chlorophylls, and a methanol fraction (MF) dominated by phenolics. While the CME and MF exhibited poor antifungal activity against Aspergillus fumigatus, Fusarium oxysporum and Colletotrichum gloeosporioides, the EAF demonstrated antifungal activity against these filamentous fungi, particularly against C. gloeosporioides. Studies with yeasts revealed that the EAF has strong effectiveness against Saccharomyces cerevisiae, Cryptococcus neoformans and Candida krusei with MICs of 8, 8 and 16 µg/mL, respectively. A combination of in vivo and in vitro studies shows that the EAF can function as a mitochondrial toxin, compromising complexes I and II activities, and as a strong inhibitor of fungal tyrosinase (Ki = 14.40 ± 4.49 µg/mL). Thus, EAF appears to be a promising candidate for the development of new multi-target fungicides.

Anti-inflammatory effects of naringenin 8-sulphonate from Parinari excelsa Sabine stem bark and its semi-synthetic derivatives.

The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases. The nuclear factor kappa ß (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development. Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive. The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity. Two compounds were detected via HPLC-DAD-ESI(Ion Trap)-MS2 analysis. The predominant compound was isolated and identified as naringenin-8-sulphonate (1), while the identity of the second compound (compound 2) could not be determined. Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway. Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1ß) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3). To explore the influence of sulphation on the anti-inflammatory properties of naringenin derivatives, we synthesized naringenin-4'-O-sulphate (4) and naringenin-7-O-sulphate (5) and evaluated their anti-inflammatory effects. Naringenin derivatives 4 and 5 did not display potent anti-inflammatory activities; however, compound 4 reduced IL-1ß production, and compound 5 diminished p65 translocation, with both exhibiting the capacity to inhibit TNF-α and IL-6 production. Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.

Allophylus africanus Stem Bark Extract Modulates the Mitochondrial Apoptotic Pathway in Human Stomach Cancer Cells.

The present work aimed to detail the mechanisms elicited by Allophylus africanus P. Beauv. stem bark extract in human stomach cancer cells and to identify the bioactives underlying the cytotoxicity. MTT reduction and LDH leakage assays allowed characterizing the cytotoxic effects in AGS cells, which were further detailed by morphological analysis using phalloidin and Hoechst 33258. Proapoptotic mechanisms were elucidated through a mitochondrial membrane potential assay and by assessing the impact upon the activity of caspase-9 and -3. The extract displayed selective cytotoxicity against AGS cells. The absence of plasma membrane permeabilization, along with apoptotic body formation, suggested that pro-apoptotic effects triggered cell death. Intrinsic apoptosis pathway activation was verified, as mitochondrial membrane potential decrease and activation of caspase-9 and -3 were observed. HPLC-DAD profiling enabled the identification of two apigenin-di-C-glycosides, vicenin-2 (1) and apigenin-6-C-hexoside-8-C-pentoside (3), as well as three mono-C-glycosides-O-glycosylated derivatives, apigenin-7-O-hexoside-8-C-hexoside (2), apigenin-8-C-(2-rhamnosyl)hexoside (4) and apigenin-6-C-(2-rhamnosyl)hexoside (5). Isovitexin-2″-O-rhamnoside (5) is the main constituent, accounting for nearly 40% of the total quantifiable flavonoid content. Our results allowed us to establish the relationship between the presence of vicenin-2 and other apigenin derivatives with the contribution to the cytotoxic effects on the presented AGS cells. Our findings attest the anticancer potential of A. africanus stem bark against gastric adenocarcinoma, calling for studies to develop herbal-based products and/or the use of apigenin derivatives in chemotherapeutic drug development.

Cartas em tempos de pandemia de Covid-19: diálogos e percursos na Residência em Saúde do Trabalhador / Letters in times of the Covid-19 pandemic: dialogues and pathways in Residency Programs in Occupational Health / Cartas en tiempos de pandemia de Covid-19: diálogos y recorridos en la Residencia en Salud del Trabajador

Este artigo visa demonstrar a potencialidade metodológica do emprego do gênero textual carta pessoal para a elaboração do portfólio crítico-reflexivo no ensino em Saúde e, consequentemente, para a formação ética e implicada na Residência Multiprofissional em Saúde do Trabalhador durante a pandemia de Covid-19. A escrita das cartas que compuseram o portfólio "Cartas em tempos de pandemia" ocorreu entre março/2020 e março/2021. As cartas, endereçadas a diversos interlocutores, permitiam refletir, de modo criativo, sobre o caminho pedagógico e percorrer processualmente as linhas de aprendizagem propostas pela Unesco: "aprender a ser, conhecer, fazer e conviver". Por esses passos metodológicos foi possível articular memórias, histórias, afetos, conceitos e práticas envolvidos no fazer em saúde, tecendo novos conhecimentos e possibilidades técnicas no trabalho da Psicologia no âmbito da Saúde do Trabalhador.(AU)

This article seeks to demonstrate the potential of the text genre personal letters as a methodology for the elaboration of critical-reflective portfolios in health education and, consequently, for ethical training in multiprofessional residency training programs in occupational health during the Covid-19 pandemic. The letters that make up the portfolio "Letters in times of pandemic" were written between March 2020 and March 2021. The letters, addressed to various interlocutors, promoted creative reflection on pedagogical pathways and allowed us to explore the processes of the lines of learning proposed by UNESCO: "learning to be, to know, to do and to live together". Using these methodological steps, it was possible to articulate the memories, stories, affects, concepts and practices involved in "doing health care", weaving together new knowledge and technical possibilities in the work of psychology in the realm of occupational health.(AU)

El objetivo de este artículo es demostrar la potencialidad metodológica del empleo del género textual carta personal para la elaboración del portafolio crítico-reflexivo en la enseñanza en salud y, consecuentemente, para la formación ética e implicada en la Residencia Multiprofesional en Salud del Trabajador durante la pandemia de Covid-19. la escritura de las cartas que compusieron el portafolio "Cartas en tiempo de pandemia" tuvo lugar entre marzo/2020 y marzo/2021. Las cartas, dirigidas a diversos interlocutores, permitían, de forma creativa, reflexionar sobre el camino pedagógico y recorrer procesualmente las líneas de aprendizaje propuestas por la Unesco: "aprender a ser, conocer, hacer y convivir". A partir de esos pasos metodológicos fue posible articular memorias, afectos, conceptos y prácticas envueltos en el quehacer en salud, tejiendo nuevos conocimientos y posibilidades técnicas en el trabajo de la psicología en el ámbito de la Salud del Trabajador.(AU)

[Analysis of the coverage of supply and of the water quality distributed in different regions of Brazil in 2019]. / Análise da cobertura de abastecimento e da qualidade da água distribuída em diferentes regiões do Brasil no ano de 2019.

Bearing in mind that universal access to sanitation services is one of the objectives of the Brazilian Plan for Basic Sanitation and one of the sustainable development goals (SDG), this research sought to evaluate the coverage of supply and of the quality of water distributed in Brazil in 2019. Data from the National Sanitation Information System (SNIS) and Water Quality for Human Consumption Surveillance Information System (Sisagua) databases were evaluated. It was found that 82% of the population has access to Water Supply Systems (WSS), this percentage only being lower in the North (58%) and Northeast (74%) regions. Most of the population is served by WSS; however, in the North region, 6.6% of the population is served by WSS and 7.2% by Collective Alternative Solution (CAS) without treatment. For the Total Coliforms parameter, 21.9% of CAS samples from the North region were outside the potability standard, while for the residual disinfectant agent, 40.6% of WSS from the Northeast region was outside this standard (with 19% of the samples having a concentration of the disinfectant agent above 5.0 mg/L, and 81% of the samples presenting concentration lower than 0.2 mg/L. The conclusion drawn is that part of the population may be vulnerable to water quality-related risks.

Tendo em vista que a universalização dos serviços de saneamento é uma das metas do Plano Nacional de Saneamento Básico (PLANSAB) e um dos objetivos de desenvolvimento sustentável (ODS), esta pesquisa objetivou-se avaliar a cobertura do abastecimento e a qualidade da água distribuída no Brasil em 2019. Foram utilizados os dados do Sistema Nacional de Informações sobre Saneamento (SNIS) e do Sistema de Informação de Vigilância da Qualidade da Água para Consumo Humano (Sisagua). Verificou-se que 82% da população foi atendida por serviços de água, sendo este percentual ainda menor considerando apenas a região Norte (58%) e Nordeste (74%). A maioria da população é atendida por Sistemas de Abastecimento de Água (SAA); entretanto, na região Norte, 6,6% da população é atendida por SAA e 7,2% por Solução Alternativa Coletiva (SAC) sem tratamentos. Para o parâmetro Coliformes totais, 21,9% das amostras de SAC da região Norte estavam fora do padrão de potabilidade, enquanto para o residual de agente desinfetante, 40,6% de SAA da região Nordeste encontravam-se fora do padrão, sendo que deste total 19% das amostras apresentavam concentração do agente desinfetante acima de 5,0 mg/L e 81% das amostras apresentavam concentrações menores que 0,2 mg/L. Conclui-se que parte da população pode estar vulnerável aos riscos relacionados à qualidade da água.

HPLC-DAD-ESI/MSn and UHPLC-ESI/QTOF/MSn characterization of polyphenols in the leaves of Neocarya macrophylla (Sabine) Prance ex F. White and cytotoxicity to gastric carcinoma cells.

Among several extracts from species from Guinea-Bissauan flora, the hydroethanol extract obtained from the leaves of gingerbread plum (Neocarya macrophylla (Sabine) Prance ex F. White.) revealed to be one of the most cytotoxic towards human gastric AGS carcinoma cells. Considering the increasing use of N. macrophylla in the food industry and the abundant biomass of agricultural wastes being generated, the identification of phenolic bioactives has been attained by HPLC-DAD-ESI/MSn and UHPLC-ESI/QTOF/MSn. Twenty-seven phenolic constituents were identified for the first time in the monotypic genus Neosartorya, 5-O-caffeoylquinic acid being detected as the major constituent (4.90 ± 0.20 mg g-1 dry extract). While 15 flavan-3-ols derivatives were determined, the extract is predominantly characterized by the occurrence of quercetin, kaempferol, apigenin and chrysoeriol glycosides. Typical apoptotic changes in gastric adenocarcinoma AGS cells upon exposure to N. macrophylla leaf extract were observed. The apoptotic cell death is mediated by the activation of the mitochondrial pathway, as loss of mitochondrial membrane potential was detected, as well as increased caspase-9 and -3 activities. The industrial relevance of this plant material, along with the data presented here on the potential anticancer effects of N. macrophylla and the efficient extraction of phenolic bioactives using water and ethanol (GRAS substance), calls for further research on the leaves as a potential functional food and/or ingredient.

Comunidade Ampliada de Pesquisa em ambiente virtual (CAP on-line) sobre trabalho e saúde docente / Expanded Community for Research in a virtual environment (CAP on-line) on teacher work and health

RESUMO A pandemia do novo coronavírus afetou a dinâmica do cotidiano escolar, obrigando os(as) professores(as) a um movimento de adaptação para dar conta das demandas impostas pelo distanciamento social. Esse cenário também atingiu as pesquisas, cujas técnicas de coletas de dados se davam de maneira presencial e tiveram que ser realizadas remotamente. O objetivo deste texto é descrever a trajetória da Comunidade Ampliada de Pesquisa, adaptada para o ambiente virtual (CAP on-line), como parte da pesquisa que buscou examinar as novas exigências do ensino remoto e suas implicações para a saúde dos(as) professores(as). Foram realizadas três oficinas com a participação de professores(as) do Ensino Fundamental e Médio. Observou-se que o processo de pesquisa tem correlações com os próprios resultados do estudo, especialmente no que se refere aos novos aprendizados trazidos pelo ensino remoto. Conclui-se que o compartilhamento de experiências de trabalho por meio da CAP on-line é uma possibilidade. No entanto, o aperfeiçoamento nos modos de interação virtual, o domínio do aparato tecnológico e o acesso a equipamentos de qualidade são elementos a serem observados para o aperfeiçoamento dessa abordagem coletiva de investigação.

ABSTRACT The new Coronavirus pandemic has affected the dynamics of everyday school life, forcing teachers to adapt to the demands imposed by social distancing. This scenario also involved the surveys, whose data collection techniques took place in person and had to be carried out remotely. This text aims to describe the trajectory of the expanded research community, adapted to the virtual format, as part of the research that sought to examine the new requirements of remote teaching and its implications for teachers' health. Three workshops were held with the participation of elementary and high school teachers. It was observed that the research process has correlations with the results of the study, especially concerning new learning brought by remote learning. It is concluded that sharing work experiences through online CAP is a possibility. However, the improvement in the modes of virtual interaction, mastery of the technological apparatus, and access to quality equipment are elements to be observed in order to improve this collective approach to investigation.

Análise da cobertura de abastecimento e da qualidade da água distribuída em diferentes regiões do Brasil no ano de 2019 / Analysis of the coverage of supply and of the water quality distributed in different regions of Brazil in 2019

Resumo Tendo em vista que a universalização dos serviços de saneamento é uma das metas do Plano Nacional de Saneamento Básico (PLANSAB) e um dos objetivos de desenvolvimento sustentável (ODS), esta pesquisa objetivou-se avaliar a cobertura do abastecimento e a qualidade da água distribuída no Brasil em 2019. Foram utilizados os dados do Sistema Nacional de Informações sobre Saneamento (SNIS) e do Sistema de Informação de Vigilância da Qualidade da Água para Consumo Humano (Sisagua). Verificou-se que 82% da população foi atendida por serviços de água, sendo este percentual ainda menor considerando apenas a região Norte (58%) e Nordeste (74%). A maioria da população é atendida por Sistemas de Abastecimento de Água (SAA); entretanto, na região Norte, 6,6% da população é atendida por SAA e 7,2% por Solução Alternativa Coletiva (SAC) sem tratamentos. Para o parâmetro Coliformes totais, 21,9% das amostras de SAC da região Norte estavam fora do padrão de potabilidade, enquanto para o residual de agente desinfetante, 40,6% de SAA da região Nordeste encontravam-se fora do padrão, sendo que deste total 19% das amostras apresentavam concentração do agente desinfetante acima de 5,0 mg/L e 81% das amostras apresentavam concentrações menores que 0,2 mg/L. Conclui-se que parte da população pode estar vulnerável aos riscos relacionados à qualidade da água.

Abstract Bearing in mind that universal access to sanitation services is one of the objectives of the Brazilian Plan for Basic Sanitation and one of the sustainable development goals (SDG), this research sought to evaluate the coverage of supply and of the quality of water distributed in Brazil in 2019. Data from the National Sanitation Information System (SNIS) and Water Quality for Human Consumption Surveillance Information System (Sisagua) databases were evaluated. It was found that 82% of the population has access to Water Supply Systems (WSS), this percentage only being lower in the North (58%) and Northeast (74%) regions. Most of the population is served by WSS; however, in the North region, 6.6% of the population is served by WSS and 7.2% by Collective Alternative Solution (CAS) without treatment. For the Total Coliforms parameter, 21.9% of CAS samples from the North region were outside the potability standard, while for the residual disinfectant agent, 40.6% of WSS from the Northeast region was outside this standard (with 19% of the samples having a concentration of the disinfectant agent above 5.0 mg/L, and 81% of the samples presenting concentration lower than 0.2 mg/L. The conclusion drawn is that part of the population may be vulnerable to water quality-related risks.

Oficinas terapêuticas com mulheres velhas: um recorte de gênero, raça e classe / Therapeutic workshops with old women: a gender, race and class approach

A velhice, apesar de ser um fenômeno biológico, é perpassada por inúmeras questões sociais que incluem marcadores de gênero, raça e classe. Tal fator traz consequências como o processo de feminização da velhice, que indica o número maior de mulheres velhas em relação ao número de homens velhos. Além disso, há mais idosas brancas do que negras e indígenas em nosso país, devido a uma menor taxa de expectativa de vida desses grupos racializados. Desse modo, torna-se importante trabalhar com sujeitos velhos, levando em conta o contexto social onde esses estão inseridos. Nesse sentido, surge o projeto "Oficinas Terapêuticas com Mulheres Velhas", na tentativa de trabalhar fatores de vulnerabilidade específicos desse público, incluindo os entrelaçamentos de raça e classe. Tal projeto foi criado por duas estudantes do curso de Psicologia, e foi realizado na Universidade Aberta à Terceira Idade (UATI), no ano de 2019. O estudo teve como base a Psicologia dos Grupos, e tem como objetivo geral, a busca por reflexões acerca do fenômeno do envelhecimento da mulher e suas particularidades, considerando as variantes de gênero, raça e classe; para isso, alguns temas como relacionamentos conjugais, racismo, medo da morte e autoestima serão discutidos, a partir de um relato de experiência feito pelas estudantes estagiárias.

Despite being a biological phenomenon, old age is permeated by numerous social issues that include markers of gender, race, and class. This aspect has consequences such as the process of feminization of old age, which indicates the higher number of older women concerning the number of older men; in addition, there are more white elderly women than black and indigenous older women in our country, due to a lower rate of life expectancy of these racialized. As such, it is important to work with these groups, considering the social context where they are inserted; in this sense, the project "Therapeutic Workshops with Old Women" appears, in an attempt to work on specific vulnerability factors of this public, including aspects of race and class. Two students of a Psychology course have created this project and was carried out at the University Open to the Third Age (UATI) in 2019. To this end, the process of creating therapeutic workshops, in their needs and possibilities, was studied, always searching to creating a project suitable for space and its participants. The study was based on Group Psychology and had as its general objective, the search for reflections about the phenomenon of the aging of women and their particularities, considering the variants of gender, race, and class; for this, some themes such as marital relationships, racism, fear of death and self-esteem will be discussed, based on an experience report made by the intern students.

Activation of caspase-3 in gastric adenocarcinoma AGS cells by Xylopia aethiopica (Dunal) A. Rich. fruit and characterization of its phenolic fingerprint by HPLC-DAD-ESI(Ion Trap)-MSn and UPLC-ESI-QTOF-MS2.

While the fruits of Xylopia aethiopica (Dunal) A. Rich. are important in African countries as a local trade product, their composition remains scarcely investigated. Phenolic fingerprint is herein delivered through HPLC-DAD-ESI(Ion Trap)-MSn and UPLC-ESI-QTOF-MS2 analysis, six cinnamoylquinic acid derivatives and twenty-four flavonoid glycosides being determined, chrysoeriol-7-O-glycosides being the main constituents. A cytotoxicity screening of twenty-eight hydroethanol extracts, obtained from a collection of Guinea-Bissauan plants, against A549 and AGS carcinoma cells, revealed the selective and potent effect towards AGS cells (IC50 = 151 × 10-3 g L-1), upon exposure to the extract from X. aethiopica fruits. Additional experiments demonstrated insignificant effect on LDH release at 151 × 10-3 g L-1, morphological analysis further suggesting induction of apoptosis. Pro-apoptotic effects were confirmed, as the extract enabled the activation of the effector caspase-3, broadening the knowledge on the anticancer mechanisms elicited by the fruits of X. aethiopica. Phenolic constituents might contribute to the cytotoxic effects, particularly via caspase-3 activation. Considering that X. aethiopica fruit is very often referred as an anticancer ingredient in Africa, but mainly the potent cytotoxicity herein recorded, our results call for additional research aiming to identify non-phenolic constituents contributing to the effects and also to further detail the anticancer mechanisms.

Cassia sieberiana DC. leaves modulate LPS-induced inflammatory response in THP-1 cells and inhibit eicosanoid-metabolizing enzymes.

ETHNOPHARMACOLOGICAL RELEVANCE: According to ethnobotanical surveys, Cassia sieberiana DC. (1825) is a particularly reputed species in African folk Medicine, namely due to the application of its leaves and roots for the treatment of diseases and symptomatology that appear to be related with an inflammatory background. In contrast with the roots of the plant, the leaves remain to be investigated, which prompted us to further detail mechanisms underlying their anti-inflammatory properties, by using in vitro models of disease. AIM OF THE STUDY: Considering its use in the amelioration and treatment of conditions that frequently underlie an inflammatory response, C. sieberiana leaves extract was prioritized amongst a collection of extracts obtained from plants collected in Guinea-Bissau. As such, this work aims to deliver experimental data on the anti-inflammatory properties of C. sieberiana leaf and to establish possible associations with its chemical composition, thus providing a rationale on its use in folk Medicine. MATERIALS AND METHODS: The chemical profile of an hydroethanol extract obtained from the leaves of the plant was established by HPLC-DAD-ESI/MSn in order to identify bioactives. The extract and its main compound were tested towards a series of inflammatory mediators, both in enzymatic and cell-based models. The capacity to interfere with the eicosanoid-metabolizing enzymes 5-lipoxygenase (5-LOX), cyclooxygenase-1 (COX-1) and -2 (COX-2) was evaluated in cell-free systems, while the effects in interleukin 6 (IL-6) and tumour necrosis factor-α (TNF-α) levels produced by THP-1 derived macrophages were assessed through ELISA. RESULTS: HPLC-DAD-ESI/MSn analysis of the extract elucidated a chemical profile qualitatively characterized by a series of anthraquinones, particularly rhein derivatives, and nine flavonols, most of which 3-O-glycosylated. Considering the concentrations of the identified compounds, quercetin was detached as the main component. Effects of the hydroethanol extract obtained from C. sieberiana leaves against key enzymes of the arachidonic acid cascade were recorded, namely a concentration-dependent inhibition against 5-LOX, at concentrations ranging from 16 to 250 µg mL-1 and a selective inhibitory action upon COX-2 (IC50 = 3.58 µg mL-1) in comparison with the isoform COX-1 (IC50 = 9.10 µg mL-1). Impact on inflammatory cytokines was also noted, C. sieberiana leaf extract significantly decreasing IL-6 levels in THP-1 derived macrophages at 250 and 500 µg mL-1. In contrast, TNF-α levels were found to be increased in the same model. Quercetin appears to partially account for the observed effects, namely due to the significant inhibitory effects on the activity of the arachidonic acid metabolizing enzymes COX-2 and 5-LOX. CONCLUSIONS: The anti-inflammatory effects herein reported provide a rationale for the use of C. sieberiana leaves in African folk practices, such as in the treatment of arthritis, rheumatism and body aches. Considering the occurrence of flavonoidic and anthraquinonic constituents, as well as the observed anti-inflammatory properties of quercetin, recorded effects must be related with the presence of several bioactives.

Kraft lignin fractionation by organic solvents: Correlation between molar mass and higher heating value.

The new concept of integrated biorefineries has significantly changed pulp and paper industries. Lignin, which until then was only burned to generate energy, is now an important raw material for new products production. Kraft lignin (KL) fractions obtained by sequential fractionation with five organic solvents. This sequence allows to extract fractions from lower molar mass to higher molar one, resulting in more homogeneous samples. Lignin's fractions were characterized by FTIR, GPC, TGA and Higher Heating Value (HHV). HHV for KL was 24966, the lowest being 17,891 (F5) and the highest being 27051 J/g (F1), inversely proportional to the molar masses of fractions. This is a very important result indicating that the lower HHV fractions can be used for certain applications, such as antioxidants, additives, polymers, among others, adding value to kraft lignin. Fractions with higher HHV could be used for energy generation in the cellulose paper industry.

Anti-inflammatory properties of Xylopia aethiopica leaves: Interference with pro-inflammatory cytokines in THP-1-derived macrophages and flavonoid profiling.

ETHNOPHARMACOLOGICAL RELEVANCE: Ethnopharmacological surveys on Guinea-Bissauan flora reveal that several species are used to treat or ameliorate the symptomatology of conditions with an inflammatory background. As such, extracts obtained from a series of plants recorded in those surveys were screened for their anti-inflammatory properties, a hydroethanolic extract obtained from the leaves of Xylopia aethiopica (Dunal) A. Rich, (Annonaceae), used on the treatment of headache, muscular pain and rheumatic pain, scoring positively and being further investigated. AIM OF THE STUDY: In order to identify species with anti-inflammatory properties, extracts were screened for their ability to interfere with LPS-induced TNF-α levels. Since significant effects were recorded upon treatment with the extract of the leaves obtained from X. aethiopica, further assays were conducted to elucidate additional mechanisms underlying its anti-inflammatory potential. Since little is known on the chemical composition of the plant, we also aimed to characterise its phenolic profile. MATERIALS AND METHODS: Interference with cytokines was evaluated by ELISA assay, through the quantification of TNF-α and IL-6 levels in the culture medium collected from LPS-activated THP-1-derived-macrophages. Inhibition of 5-lipoxygenase was assessed based on the oxidation of linoleic acid to 13-hydroperoxylinoleic acid. Characterization of the phenolic profile was attained by HPLC-DAD. RESULTS: Evaluation of TNF-α levels in LPS-challenged THP-1 macrophages evidenced a significant inhibition (>90%) upon treatment with the hydroethanolic extract obtained from X. aethiopica leaves at a concentration of 500 µg/mL. Additional anti-inflammatory effects were recorded, including a significant decrease on IL-6 levels at 250 and 500 µg/mL. The extract proved to be active towards 5-LOX, leading to significant inhibition at concentrations ranging from 16 to 250 µg/mL (IC50 = 85 µg/mL). Phenolic profiling allowed the identification and quantitation of eight constituents, including caffeoylquinic acids (1-3), mono-O-glycosylated flavonols (5-8), and the mono-O-glycosyl flavone luteolin-7-O-glucoside (4). The main phenolic constituent, kaempferol-3-O-rutinoside (8), was found to significantly contribute to the anti-inflammatory effects, namely through the inhibition of 5-LOX. However, no effects on the decrease of TNF-α and IL-6 levels caused by this phenolic compound were found. CONCLUSION: The anti-inflammatory effects of X. aethiopica leaves are demonstrated experimentally, thus substantiating its use in folk Medicine. Relevantly, the observed anti-inflammatory properties can stimulate further studies in order to fully unveil the therapeutic potential of the plant, namely as a source of phenolic compounds with a significant ability to interfere with conventional inflammatory targets.

Flavonoid Composition of Salacia senegalensis (Lam.) DC. Leaves, Evaluation of Antidermatophytic Effects, and Potential Amelioration of the Associated Inflammatory Response.

Predominantly spread in West Tropical Africa, the shrub Salacia senegalensis (Lam.) DC. is known because of its medicinal properties, the leaves being used in the treatment of skin diseases. Prompted by the ethnomedicinal use, a hydroethanolic extract obtained from the leaves of the plant was screened against a panel of microbial strains, the majority of which involved in superficial infections. The extract was found to be active against the dermatophytes Trichophyton rubrum and Epidermophyton floccosum. Notable results were also recorded regarding the attenuation of the inflammatory response, namely the inhibitory effects observed against soybean 5-lipoxygenase (IC50 = 71.14 µg mL-1), no interference being recorded in the cellular viability of RAW 264.7 macrophages and NO levels. Relevantly, the extract did not lead to detrimental effects against the keratinocyte cell line HaCaT, at concentrations displaying antidermatophytic and anti-inflammatory effects. Flavonoid profiling of S. senegalensis leaves was achieved for the first time, allowing the identification and quantitation of myricitrin, three 3-O-substituted quercetin derivatives, and three other flavonoid derivatives, which may contribute, at least partially, to the observed antidermatophytic and anti-inflammatory effects. In the current study, the plant S. senegalensis is assessed concerning its antidermatophytic and anti-inflammatory properties.

Leaves and stem bark from Allophylus africanus P. Beauv.: An approach to anti-inflammatory properties and characterization of their flavonoid profile.

The small tree Allophylus africanus, widespread in the African continent, has long been considered valuable, as noted by the number of reports on their multiple medicinal uses. With this work, we aimed to extend the current, and so far restricted, knowledge on the chemical composition of the plant, particularly as source of flavonoids, as well as to assess its potential anti-inflammatory properties. The chemical characterization of the aqueous extract obtained from the leaves allowed the identification and quantitation of 30 flavones, predominantly apigenin derivatives, but also four luteolin derivatives, while the stem bark extract was solely characterized by apigenin di-C-glycosides and mono-C-glycosides-O-glycosylated. Strong inhibitory effects towards 5-lipoxygenase were observed with the aqueous extracts obtained from the leaves and stem bark, with IC50 values of 41.28 and 107.77 µg mL-1, respectively. Both extracts were also found to reduce NO levels in LPS-challenged RAW 264.7 macrophages, without noticeable cytotoxicity. The flavonoid profile of the plant is disclosed for the first time, allowing the identification of several molecules that may contribute to mitigate the inflammatory response. Jointly, with the current study the anti-inflammatory use of the leaves and stem bark is partially validated.

Genetic control of innate and acquired immunity

Host defense against infection depends on both specific and nonspecific mechanisms.The lines of mice genetically selected for high (H) or low (L) antibody responsiveness and for the maximal (AIR max.) or minimal (AIR min.) acute inflammatory response, in which the opposite extreme potentialities have been clearly defined, ofter an appropriate model for investigation of the major genetic and environmental factors of resistance to infections. The alternative advantagens of the extreme phenotypes such as efficacy of specific and nonspecific immunity in natural populations are discussed.